There is only one, single-lap course available, but it has different themes, which pulls elements from courses within Mario Kart Arcade GP DX. Gliders are also present, and are used similarly to Mario Kart Arcade GP DX.

Also, instead of using Item Boxes, the game uses red, yellow, and green balloons to store items. For example, to throw a Green Shell, one must make a throwing motion with their open palm. In addition, items are thrown by making hand movements, which the game registers by an attachment to the player's wrist. Similar to the prior arcade Mario Kart games, the game is controlled via a steering wheel and acceleration and brake pedals. Unlike the prior Mario Kart games, Mario Kart Arcade GP VR is played through use of a virtual reality headset and in first person.